Human ASMA (Anti Smooth Muscle Antibody) ELISA Kit (HUFI04763)

The ASMA ELISA from Assay Genie is an in vitro diagnostic immunoassay used to quantify circulating anti-smooth muscle autoantibodies, which serve as serological markers for autoimmune hepatitis type 1 and related chronic liver disorders. The capture antibodies recognize contractile filament proteins such as F-actin, desmin, and vimentin, allowing investigators to evaluate titers against cytoskeletal components in patient specimens. The kit provides human-specific detection with reactivity toward smooth muscle autoantibody targets.

The kit is supplied ready-to-use with pre-coated microplate wells, calibrated standards, and detection reagents sufficient for 96 individual determinations in a single run. For best results, serum samples should be separated promptly, aliquoted to avoid repeated freeze-thaw cycles, and stored refrigerated until processing. The assay performs reliably across a broad analytical range, with intra-assay precision typically below 10% CV, supporting reproducible quantification across multiple runs.