Human ASAH2/Neutral Ceramidase PharmaGenie ELISA Kit (SBRS0283)

The Human ASAH2/Neutral Ceramidase ELISA Kit from Assay Genie enables quantitative measurement of neutral ceramidase, a key enzyme in sphingolipid metabolism that hydrolyzes ceramide into sphingosine and free fatty acids at neutral pH. ASAH2 is anchored to the plasma membrane and Golgi apparatus, where it modulates ceramide-to-sphingosine balance, influencing cell proliferation, apoptosis, inflammatory signaling, and gut barrier homeostasis.

Validated specifically for the human ASAH2 isoform and exhibiting no significant cross-reactivity with the murine or rat orthologs, this assay is used by researchers studying metabolic disorders, inflammatory bowel disease, colorectal cancer, and obesity-related pathways to monitor ASAH2 levels in conditioned media and patient-matched serum specimens. Each kit contains a precoated microplate, capture antibody, detection reagent, standards, and substrate solution sufficient for 96 assays. Reconstituted reagents should be stored at -20°C in aliquots to limit freeze-thaw cycles, and the strip wells remain stable at 4°C until the expiration date printed on the label.

Assay Genie supports the kit with a detailed protocol, and the PharmaGenie reagent line is intended for research use only, in vitro laboratory applications, and pharmaceutical drug discovery workflows investigating ceramide-modulating therapeutics.