Human Arylalkylamine-N-Acetyltransferase (AANAT) ELISA Kit

Arylalkylamine-N-acetyltransferase (AANAT) is the rate-limiting enzyme in melatonin biosynthesis, converting serotonin to N-acetylserotonin in the pineal gland. This immunoassay from Assay Genie enables quantitative detection of human AANAT protein in tissue homogenates, cell lysates, and similar sample matrices, supporting research into circadian rhythm biology, seasonal photoperiod responses, and neuroendocrine signaling.

The kit is supplied as a complete reagent set with a pre-coated microplate sufficient for 96 determinations. Researchers apply diluted samples and standards to the plate, perform the immunoassay workflow, and measure absorbance to determine AANAT concentration in each specimen. The protocol is intended for research use only and is compatible with standard plate-reader instrumentation found in most immunology and neuroscience laboratories.

Typical applications include profiling AANAT expression in pineal tissue, tracking enzyme levels in cell culture models of circadian oscillation, and comparing AANAT abundance between healthy and disease-state samples in endocrinology studies.