Human ARMET/MANF ELISA Kit

Assay Genie provides a high-quality Sandwich ELISA Kit for the quantitative measurement of ARMET (Arginine-Rich, Mutated in Early Stage Tumors) protein in human samples. The kit uses a colorimetric detection method in a double-antibody sandwich ELISA format, designed to deliver accurate and consistent results across a diverse array of research applications. ARMET is a pivotal protein implicated in key biological processes such as cell survival, proliferation, and metabolism, and its dysregulation has been linked to various diseases including cancer, neurodegenerative disorders, and metabolic syndromes, making it a vital biomarker for investigating these pathologies.

The kit is validated for use with human serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. It offers a sensitivity of 9.375 pg/mL with a working range of 15.625–1000 pg/mL. Reported linearity for serum (n=5) spans 86–101%, 87–103%, and 85–102% at 1:2, 1:4, and 1:8 dilutions respectively; EDTA plasma shows 83–98%, 84–101%, and 84–101%; heparin plasma shows 82–97%, 81–99%, and 82–96%. Mean recovery is 96% for serum, 95% for EDTA plasma, and 95% for heparin plasma (ranges 93–100%, 88–99%, and 89–101% respectively).

The assay workflow includes a 90-minute primary incubation at 37°C, a 60-minute biotinylated detection antibody step, a 30-minute HRP-Streptavidin (SABC) incubation, and 10–20 minutes of TMB substrate development in the dark, with absorbance read at 450 nm after stop solution is added. The kit corresponds to UniProt P55145 and is intended for research in the metabolism area. Components should be stored at 2–8°C, with the kit stable for 12 months under these conditions.