Human ARL4 ELISA Kit (HUFI04634)

ARL4 belongs to the ADP-ribosylation factor-like (Arl) subfamily of small GTP-binding proteins, which regulate membrane trafficking, cytoskeletal dynamics, and signal transduction through pathways such as Wnt/β-catenin. Altered ARL4 expression has been associated with several human cancers and with disorders of cellular proliferation, making quantitative detection a valuable tool for oncology research, cell biology studies, and biomarker discovery programs.

The plate arrives pre-coated with a capture antibody specific to human ARL4. Diluted patient specimens, along with a series of calibrator standards, are loaded onto the plate and incubated. A biotinylated detection antibody and streptavidin-HRP conjugate are added sequentially with washing between steps. Substrate is then dispensed, the enzymatic reaction produces a colored product, the reaction is terminated, and absorbance is recorded for quantification against the standard curve.

For reliable results, collect samples under standard clinical protocols, aliquot them to limit repeated freeze-thaw cycles, and store refrigerated for short-term use or frozen at -80 degrees C for extended archiving. Bring all kit components to room temperature before initiating the run and read the plate promptly after substrate addition to keep timing consistent across wells.

This Assay Genie kit is configured for research use only and supports detection of human ARL4 in serum-derived or plasma-derived specimens. It is well suited to comparative profiling between patient cohorts and healthy controls, screening for pathway activation, and validation of ARL4 as a candidate biomarker in translational research.