Human ARID4B ELISA Kit

Manufactured by Assay Genie, this ELISA kit is designed for the quantitative measurement of human ARID4B in research samples, using a sandwich ELISA, double antibody detection method with colorimetric readout. The assay is reactive with human samples and accepts a range of liquid sample types, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, making it suitable for diverse experimental workflows.

The kit has a reported sensitivity of 0.188 ng/mL and a working range of 0.313-20 ng/mL, and it should be stored at 2-8°C where it remains stable for 12 months. It falls within the Epigenetics and Nuclear Signaling research area and is associated with Uniprot entry Q4LE39.

The workflow involves reagent and plate preparation, primary incubation at 37°C for 90 minutes, detection antibody binding for 60 minutes, HRP-streptavidin binding for 30 minutes, TMB color development in the dark for 10-20 minutes, and a stop reaction with absorbance read at 450 nm. Reported recovery averages are 96% in serum, 95% in EDTA plasma, and 99% in heparin plasma, supporting reliable analyte quantification across the indicated sample matrices.