Human Arginase (Arg) ELISA Kit

The Arginase enzyme is a manganese-dependent metalloenzyme (EC 3.5.3.1) that catalyzes the hydrolysis of L-arginine to L-ornithine and urea, occupying a central position in the hepatic urea cycle and in immune cell regulation through the M1 versus M2 macrophage polarization that shapes inflammatory and wound-healing responses. The Assay Genie Human Arginase ELISA Kit enables quantitative measurement of this enzyme across a 96-assay microplate layout, supporting research programs in liver biology, vascular pathophysiology, tumor immune evasion, and inherited or acquired disorders of arginine metabolism.

Researchers can apply the kit to serum samples, plasma samples, tissue homogenates, cell lysates, and cell culture supernatants, with each well plate position intended for a single use. The absorbance-based read-out is compatible with standard laboratory plate readers, and the human-specific reactivity makes the assay appropriate for translational research, patient-derived sample screening, and cell-based models of L-arginine handling. In experimental contexts, the kit supports investigation of arginase isoform expression (cytosolic Arg1 versus mitochondrial Arg2), the competitive crosstalk between arginase and nitric oxide synthase for their shared L-arginine substrate, and downstream effects on collagen synthesis, T-cell proliferation, smooth-muscle relaxation, and tissue repair. Reagents should be refrigerated and protected from light to preserve enzymatic activity through the stated shelf life.