Human ARFGEF1 (Brefeldin A-inhibited guanine nucleotide-exchange protein 1) ELISA KIT (HUFI05475)

ARFGEF1, also known as BIG1, encodes a large ~200 kDa guanine nucleotide-exchange factor that harbors a Sec7 catalytic domain responsible for activating ARF1 and ARF3 GTPases. This Brefeldin A-inhibited protein localizes to the trans-Golgi network and participates in vesicular trafficking, Golgi maintenance, and nuclear transcriptional regulation through P300/CBP co-activator interactions. Researchers investigating intracellular transport, organelle biogenesis, and small GTPase signaling pathways use this Assay Genie kit to quantify endogenous human ARFGEF1 levels in experimental specimens, supporting studies in cell biology, oncology, neuroscience, and developmental biology where altered ARFGEF1 expression has been linked to periventricular nodular heterotopia and tumor progression.