Human Arachidonate 5-lipoxygenase (ALOX5) ELISA Kit

A high-quality Sandwich ELISA from Assay Genie designed for the precise quantitative measurement of human Arachidonate 5-lipoxygenase (ALOX5) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The kit employs a colorimetric detection method and is reactive with human samples.

ALOX5 is an enzyme involved in the metabolism of arachidonic acid, playing a key role in the synthesis of leukotrienes and inflammation regulation. Dysregulation of ALOX5 has been linked to inflammatory disorders, asthma, and other health conditions, making it a valuable biomarker for understanding disease mechanisms and potential therapeutic interventions. The protein, known by synonyms 5-LO and LOG5, catalyzes the first step in leukotriene biosynthesis. It interacts with ALOX5AP, LTC4S and COTL1, with the COTL1 interaction required for stability and efficient catalytic activity.

Reported performance specifications include a detection range of 0.156-10ng/mL and a sensitivity of 0.078ng/mL. Precision is reported with an Intra CV of 4.6% and Inter CV of 7.5%. Recovery values are documented at an average of 87% (range 81-93%) for serum and 89% (range 83-95%) for plasma. The assay is specific for natural and recombinant human Arachidonate 5-lipoxygenase and is listed under cardiovascular research applications. The product is intended for research use only.