Human Arachidonate-5-Lipoxygenase (ALOX5) ELISA Kit

From Assay Genie, the immunoassay enables quantitative measurement of human ALOX5, the arachidonate 5-lipoxygenase enzyme that catalyzes conversion of arachidonic acid into leukotriene signaling mediators. Because ALOX5 sits at the head of the leukotriene biosynthesis pathway, the kit is applied across inflammation research, including studies of asthma, allergic airway disease, chronic inflammatory disorders, atherosclerosis, and oncology models in which 5-LOX activity contributes to tumor progression.

Each kit ships as a complete pack containing pre-coated microplate wells, biotinylated detection antibody, enzyme conjugate, wash buffer, substrate solution, and recombinant human ALOX5 standard for construction of the standard curve. Researchers add standards and prepared samples to the wells, incubate, wash, and develop the color reaction for absorbance measurement on a standard microplate reader. The assay supports in vitro analysis of multiple human specimen types, including tissue homogenates, cell lysates, cell culture supernatants, and standard blood-derived fractions. Reagents should be refrigerated upon arrival; reconstituted standards should be aliquoted to limit freeze-thaw cycles and preserve activity. The single-use plate format supports parallel processing across a full plate within a single experimental run.