Human Arachidonate-15-Lipoxygenase (ALOX15) ELISA Kit

The 15-lipoxygenase enzyme encoded by the ALOX15 gene catalyzes the stereospecific oxygenation of arachidonic acid, generating 15-hydroperoxyeicosatetraenoic acid (15-HPETE) that feeds into the lipoxin and resolvin pathways controlling inflammatory resolution. Quantitative measurement of ALOX15 protein abundance in cell lysates and tissue homogenates supports research into ferroptosis, atherosclerotic plaque biology, airway inflammation, and eicosanoid signaling. This immunoassay from Assay Genie is configured as a complete 96-assay kit containing pre-coated microwell strips, lyophilized recombinant human ALOX15 reference standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop reagent. Sample handling recommendations include homogenizing fresh or frozen tissue in non-denaturing lysis buffer supplemented with protease inhibitors, clarifying by centrifugation, and diluting cleared lysates into the working range of the assay. Researchers may also adapt the protocol for clarified cell culture supernatants or other protein-containing biological samples after verifying matrix compatibility on a small pilot run. Absorbance is recorded with a standard microplate reader, and unknown concentrations are interpolated from a multi-point standard curve generated with the supplied reference material. Reagents should be equilibrated to room temperature before each run, stored according to the label, and protected from light to preserve enzymatic conjugate activity across the plate.