Human Arachidonate-12-Lipoxygenase (ALOX12) ELISA Kit

Quantitative measurement of arachidonate-12-lipoxygenase (ALOX12) in human research samples is supported by this ELISA kit from Assay Genie. ALOX12 is a non-heme iron-containing lipoxygenase that catalyzes the oxygenation of arachidonic acid into 12-hydroperoxyeicosatetraenoic acid (12-HPETE), a precursor of bioactive lipid mediators implicated in platelet activation, atherosclerosis progression, and several cancer signaling pathways.

Validated for tissue homogenates and cell lysates, the microplate-format kit delivers reagents sufficient for 96 assays per plate. A chromogenic substrate produces a measurable product after incubation, and absorbance readings quantify ALOX12 levels in the sample. Intended for research use only, the kit finds application in studies of cardiovascular pathophysiology, inflammatory disease mechanisms, and tumor microenvironments.

Kit components should be refrigerated upon arrival and equilibrated to room temperature before each run. Researchers commonly pair ELISA findings with western blot confirmation when characterizing ALOX12 expression changes across in vitro cell-based and tissue-derived samples.