Human Aquaporin-1 (AQP1) ELISA Kit

A sandwich ELISA from Assay Genie for the quantitative measurement of human Aquaporin-1 (AQP1) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The kit is reactive with human samples and detects natural and recombinant human Aquaporin-1. For research use only.

The assay reports a detection range of 0.156–10 ng/mL with a sensitivity of 0.04 ng/mL. Precision is given as an intra-assay CV of 4.9% and inter-assay CV of 7.8%. Linearity was assessed at 1:2, 1:4, 1:8 and 1:16 dilutions for serum (106–118%, 96–108%, 109–119%, 107–117%), EDTA plasma (91–103%, 99–109%, 110–122%, 89–102%) and heparin plasma (98–110%, 97–107%, 82–92%, 88–98%). Recovery averaged 97% for serum (range 91–103%) and 99% for plasma (range 93–105%).

Aquaporin-1 forms a water-specific channel that gives the plasma membranes of red cells and kidney proximal tubules high permeability to water, permitting water to move in the direction of an osmotic gradient. It is a homotetramer that localises to the cell membrane as a multi-pass membrane protein, interacts with EPHB2 in endolymph production, and has been identified in a complex with STOM. Documented synonyms include Aquaporin-CHIP, Urine water channel, Water channel protein for red blood cells and kidney proximal tubule, AQP-1 and CHIP28.