Human Apoptotic Peptidase Activating Factor 1 (APAF1) ELISA Kit

Measure human APAF1 (Apoptotic Peptidase Activating Factor 1), a 130 kDa cytosolic adapter protein central to the intrinsic apoptosis pathway, in tissue homogenates and cell lysates with this quantitative immunoassay from Assay Genie.

APAF1 oligomerizes into the apoptosome complex with cytochrome c and procaspase-9 to drive caspase-9 activation and the downstream caspase cascade. This places APAF1 at a critical control point in programmed cell death, supporting research into tumor biology, neurodegeneration, chemotherapy-induced cytotoxicity, and developmental cell elimination. The reagent is intended for research use only.

The kit provides reagents for 96 assays on a microplate, with a colorimetric readout proportional to APAF1 concentration in human research samples. Researchers use it to monitor apoptosome assembly, compare basal versus stimulated apoptosis, and quantify pathway activity in disease models and pharmacological screens.