Human Apoptosis-associated speck-like protein containing a CARD (PYCARD) ELISA Kit

A high-quality Sandwich ELISA Kit from Assay Genie for the precise quantification of human Apoptosis-associated speck-like protein containing a CARD (PYCARD) levels in biological samples. The assay is reactive with human samples and has been validated for serum, plasma, and cell culture supernatants, with the manufacturer also listing tissue homogenates and other biological fluids as suitable sample types. Detection is by ELISA, with a stated range of 0.156-10ng/mL and a sensitivity of 0.078ng/mL.

The kit is reported to recognise natural and recombinant human PYCARD, with an intra-assay CV of 4.9% and an inter-assay CV of 7.3%. PYCARD, also known as ASC, hASC, CARD5, TMS1, Caspase recruitment domain-containing protein 5, PYD and CARD domain-containing protein, or Target of methylation-induced silencing 1 (UniProt Q9ULZ3), functions as a key mediator in apoptosis and inflammation, including activation within inflammasomes such as NLRP2, NLRP3, AIM2 and others, and is localised to the cytoplasm, endoplasmic reticulum, mitochondria and nucleus. The kit is intended for research use, supporting studies in areas such as cancer and the investigation of autoinflammatory, infectious and immune-related diseases.