Human Apolipoprotein H/ApoH ELISA Kit

Beta-2-glycoprotein I (β2-GPI), also known as Apolipoprotein H, is a 50 kDa plasma glycoprotein that circulates at approximately 200 µg/mL in healthy adults and acts as an autoantigenic cofactor in antiphospholipid syndrome. This quantitative ELISA from Assay Genie supports investigation into recurrent pregnancy loss, autoimmune thrombosis, atherosclerosis progression, and lipid-metabolism disorders. Each kit provides materials for 96 reactions in a microplate layout, with calibrators, detection antibody, wash buffer, substrate, and stop solution supplied lyophilized or pre-diluted as appropriate. All incubations are carried out at room temperature, and reconstituted standards tolerate multiple freeze-thaw cycles when aliquoted into single-use vials. The protocol is intended for research use only, accommodates small-volume samples, and supports ApoH quantification from human-derived circulatory specimens with minimal consumption of precious material. Researchers studying autoimmune vasculopathy, placental insufficiency, or hepatic dysfunction can integrate this ELISA into workflows that already include western blot confirmation, PCR validation of candidate genes, or lipoprotein profiling by electrophoresis and chromatography.