Human Apolipoprotein A-I (APOA1) ELISA Kit

This sandwich ELISA from Assay Genie is designed for the quantitative detection of human Apolipoprotein A-I (APOA1), a key component of high-density lipoprotein (HDL) involved in lipid metabolism and cholesterol transport. APOA1 is recognised for its anti-inflammatory and antioxidant properties and serves as a biomarker in research on cardiovascular health and metabolic disorders.

The assay uses a double-antibody sandwich format with colorimetric readout and is validated for human serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Reported sensitivity is 0.188 ng/mL with an assay range of 0.313–20 ng/mL. Manufacturer data show linearity of 85–105% for serum, 82–101% for EDTA plasma, and 81–100% for heparin plasma across serial dilutions, with recovery ranges of 85–105% (serum), 86–97% (EDTA plasma) and 86–104% (heparin plasma). Reagents are stored at 2–8°C for up to 12 months.