Human APOA5/Apolipoprotein A5 ELISA Kit

Manufactured by Assay Genie, this sandwich double-antibody ELISA uses a colorimetric readout at 450 nm and is intended for the quantitative determination of human apolipoprotein A5 in research applications. The target protein plays a crucial role in regulating triglyceride levels in the blood, and abnormal concentrations have been linked to dyslipidemia, obesity, and cardiovascular diseases. The kit supports studies of lipid metabolism and cardiovascular health.

Reported sensitivity is 0.469 ng/mL with a working range of 0.781–50 ng/mL. Sample types include serum, plasma, cell culture supernatants, cell or tissue lysates, and other liquid samples. Linearity in EDTA plasma spans 82–98% across serial dilutions, with average recovery of 95%; heparin plasma shows 81–99% linearity and 96% average recovery. The procedure uses a biotin-labeled detection antibody with HRP-streptavidin conjugate and TMB substrate. Kit components are stored at 2–8°C and remain stable for 12 months under these conditions.