Human ApoA PharmaGenie ELISA Kit (SBRS0267)

ApoA (apolipoprotein A) is the principal protein constituent of high-density lipoprotein (HDL) and a key driver of reverse cholesterol transport, the process by which excess cholesterol is moved from peripheral tissues back to the liver for excretion. ApoA-I alone accounts for roughly 70% of HDL protein mass, and quantitative measurement of circulating ApoA is widely used in cardiovascular and metabolic research, including studies of HDL function, atherosclerosis, dyslipidemia, and the metabolic syndrome.

The Assay Genie Human ApoA PharmaGenie ELISA Kit is supplied as a research-use-only immunoassay configured for 96 individual determinations per plate. It is validated to detect human ApoA in plasma specimens, serum specimens, and cell culture supernatant, supporting both in vitro cell-based experiments and clinical sample analysis in studies focused on human lipoprotein metabolism.

Each kit includes a pre-coated assay plate, biotinylated detection antibody, streptavidin-HRP conjugate, a set of calibrators for standard curve generation, assay and wash buffers, substrate solution, and stop solution. The procedure follows a capture-and-detection workflow in which ApoA present in standards and samples is immobilized on the plate, recognized by the detection antibody, and visualized through an enzymatic reaction read on a standard microplate absorbance instrument.

For best results, store unopened kit components refrigerated until first use, protect the substrate from light, allow all reagents to reach room temperature before running the assay, and avoid repeated freeze-thaw cycles of diluted antibody and conjugate working solutions. The kit is intended for research use only and is not validated for clinical diagnostic applications.