Human Apelin-36/AP36 ELISA Kit

This competitive ELISA kit from Assay Genie is specifically designed for the precise measurement of Apelin-36 levels in human biological samples. It employs a colorimetric detection method in a competitive assay format with the plate coated with antigen, and is reactive with human samples including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit is intended for research applications exploring the role of Apelin-36, a peptide involved in cardiovascular function, metabolic regulation, and energy homeostasis, where it serves as a valuable biomarker linked to conditions such as obesity, diabetes, and cardiovascular diseases.

The assay delivers a sensitivity of 28.125 pg/mL with a detection range of 46.875–3000 pg/mL. Linearity has been validated in serum (n=10), EDTA plasma (n=10), and heparin plasma (n=10) across dilution ratios of 1:2, 1:4, and 1:8, with serum ranging 85–105%, EDTA plasma ranging 83–101%, and heparin plasma ranging 83–100%. Recovery has been assessed with serum (n=10) at 86–104% (average 93%), EDTA plasma (n=10) at 89–100% (average 91%), and heparin plasma (n=10) at 90–104% (average 96%). The kit should be stored sealed at 2–8°C and must not be cryopreserved.