Human ANXA4 ELISA Kit

Assay Genie offers a sandwich ELISA designed for the quantitative measurement of Annexin A4 (ANXA4) in human samples, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types. The kit uses a colorimetric, double-antibody sandwich format and is suited to research in cell biology, particularly studies relating to inflammation, cell proliferation, and apoptosis.

Reported performance includes a sensitivity of 46.875 pg/mL and a working range of 78.125-5000 pg/mL. Linearity has been characterised across 1:2, 1:4 and 1:8 dilutions in serum (85-101%), EDTA plasma (82-100%) and heparin plasma (81-100%). Average recovery is 97% in serum (range 85-103%), 90% in EDTA plasma (range 86-98%) and 98% in heparin plasma (range 90-104%). The kit includes a biotin-labelled detection antibody, HRP-streptavidin conjugate, TMB substrate and the buffers required to run the colour-development protocol. Reagents are stored at 2-8°C and remain stable for 12 months.