Human Antithrombin 3/SERPINC1 PharmaGenie ELISA Kit (SBRS0286)

Quantify human antithrombin III, the SERPINC1-encoded serine protease inhibitor that regulates thrombin and factor Xa activity, with this immunoassay from Assay Genie. The kit supports research workflows in thrombophilia screening, disseminated intravascular coagulation studies, hepatic synthesis assessment, sepsis-associated coagulopathy, and pharmacodynamic evaluation of heparin cofactor activity in patient samples.

Each pack supplies a pre-coated microplate, lyophilized reference standard, biotinylated detector antibody, streptavidin-HRP conjugate, wash buffer, and substrate solution for 96 determinations read on a standard absorbance plate reader. Sample matrices include anticoagulated plasma, with parallel protocols for serum-based quantification and clarified cell culture supernatant harvested from in vitro cell-based studies.

Typical applications include monitoring recombinant antithrombin concentrate pharmacokinetics, profiling patient cohorts for inherited antithrombin deficiency, benchmarking antithrombin expression in hepatic cell models, and characterizing antithrombin consumption during extracorporeal circulation studies. Components arrive in a sealed pouch with desiccant and remain stable when stored refrigerated between uses; reconstituted standards should be frozen in single-use aliquots to limit freeze-thaw cycles prior to each assay run.