Human Antigen KI-67 (MKI67) ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA Kit is designed for the quantitative measurement of MKI67 (Ki-67) levels in human samples. The Ki-67 protein is a well-known marker of cell proliferation, and its expression level correlates with cell proliferation rate, making it a valuable biomarker in oncology research. Suitable for serum, plasma, and cell culture supernatants, the kit offers versatility for research applications studying cell proliferation dynamics, assessing treatment responses, and exploring potential therapeutic targets in cancer and other diseases.

The assay uses a colorimetric sandwich ELISA format and is reactive with human samples, with specificity for natural and recombinant human Proliferation marker protein Ki-67 (UniProt P46013). Performance specifications include a detection range of 0.312-20ng/mL and a sensitivity of 0.1ng/mL. Precision is reported as an intra-assay CV of 4.9% and inter-assay CV of 6.7%. Recovery rates are 98% (range 92-104%) for serum and 100% (range 94-106%) for plasma.

For research use only, the kit is relevant to studies within the epigenetics research area, as Ki-67 is required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly.