Human anti-HSPA5 (anti-Heat shock protein family A member 5) ELISA Kit (HUFI08015)

Designed for the in vitro quantitative determination of Human anti-HSPA5 (anti-Heat shock protein family A member 5) concentrations in serum, plasma, and other biological fluids, this Indirect - Biotin ELISA Kit from Assay Genie uses a colorimetric detection method. HSPA5, also known as BiP (Immunoglobulin Heavy Chain-Binding Protein) and GRP78, is a 78 kDa glucose-regulated chaperone of the HSP70 family that plays a critical role in protein folding and assembly within the endoplasmic reticulum during cellular stress.

The kit reports a sensitivity of < 0.234 ng/ml and an assay range of 0.391-25 ng/ml, with precision values of Intra-Assay CV<8% and Inter-Assay CV<10%. The assay is read on a microplate reader using a 450 nm wavelength filter. Components are stored at 4°C for 6 months, and the kit is intended for Research Use Only.

Dysregulation of HSPA5 has been linked to numerous diseases, including cancer, diabetes, and neurodegenerative disorders, highlighting its value as a biomarker. HSPA5 (UniProt: P11021) is required for endoplasmic reticulum integrity and stress-induced autophagy and plays a central role in regulating the unfolded protein response (UPR).