Human anti-2019 nCoV (S)IgG ELISA Kit (HUFI03464)

Assay Genie's anti-spike (S) IgG immunoassay uses an indirect biotin-streptavidin-HRP workflow to quantitatively measure human IgG antibodies specific to the SARS-CoV-2 spike glycoprotein. Recombinant S antigen is pre-coated on the microplate, where circulating anti-S IgG from patient specimens is captured and revealed with a biotinylated anti-human IgG secondary antibody plus streptavidin-HRP conjugate, producing a colour signal proportional to antibody titre. Researchers apply this 96 Assays kit format for seroprevalence studies, vaccine-response monitoring, and confirmation of prior COVID-19 infection, with the spike-specific capture antibody helping reduce cross-reactivity against endemic human coronaviruses such as OC43 and 229E. Each run uses a small sample volume of 10 μL loaded in duplicate, with all reagents refrigerated at 4°C upon arrival and the standard curve, wash buffer, and conjugate handled according to the supplied protocol.