Human Anti-2019 nCoV (N+S)IgM ELISA Kit (HUFI03452)

This indirect biotin-streptavidin immunoassay captures IgM antibodies directed against both the nucleocapsid (N) and spike (S) antigens of SARS-CoV-2, supporting identification of recent or active immune responses. The plate-based workflow requires approximately 100 uL of patient specimen per well, with streptavidin-HRP amplification to strengthen the chromogenic signal. Assay Genie supplies pre-coated wells, biotinylated detection reagent, standards, wash concentrate, TMB substrate, and stop solution within the kit for a complete microplate run. Component vials should be kept refrigerated and shielded from light to preserve binding activity through the stated shelf life, with repeated freeze-thaw cycles of the conjugate avoided. The configuration is intended for research use only, making it appropriate for seroprevalence studies, vaccine response monitoring, and comparative evaluation of convalescent versus immunized cohorts where dual N+S antigen coverage improves detection of early IgM seroconversion.