Human Anti-2019 nCoV (N+S)IgG ELISA Kit (HUFI03451)

This Indirect - Biotin ELISA Kit from Assay Genie detects human IgG antibodies against the nucleocapsid and spike proteins of SARS-CoV-2, supporting seroprevalence surveys, vaccine response monitoring, and convalescent serum screening. The dual N + S antigen coating captures a broader antibody repertoire than single-target assays, reducing the chance of false negatives when circulating variants shift epitope immunodominance.

Each kit includes a pre-coated 96-well microplate, biotinylated secondary antibody, streptavidin-HRP conjugate, TMB substrate, and calibrated standards. The protocol requires a 10 µL sample of serum or plasma per well, with biotin-amplified signal development for improved sensitivity at low antibody titres. Store unopened kits at 2-8 degrees C, protect from light, and avoid freeze-thaw of the coated plate.