Human Ankyrin repeat domain-containing protein 30A (ANKRD30A) ELISA Kit

A sandwich ELISA kit from Assay Genie for the quantitative measurement of human Ankyrin repeat domain-containing protein 30A (ANKRD30A), also referred to by the synonym Serologically defined breast cancer antigen NY-BR-1. The assay uses a colorimetric detection method and is reactive with human samples, including serum, plasma, and cell culture supernatants. The manufacturer describes ANKRD30A as a DNA-binding transcription factor containing ankyrin repeat domains that participates in protein-protein interactions, signal transduction, cell growth, differentiation, and development, with altered expression reported in association with breast cancer progression. The kit is intended for research use only and is validated for specificity toward natural and recombinant human ANKRD30A.

Performance figures quoted by the manufacturer include a detection range of 0.156-10 ng/mL, a sensitivity of 0.089 ng/mL, an intra-assay CV of 4.7%, and an inter-assay CV of 8.3%. Reported recovery is 97% (range 91-103%) in serum and 99% (range 93-105%) in plasma. Linearity has been assessed in serum, EDTA plasma, and heparin plasma across serial dilutions. The assay is built around a quantitative sandwich ELISA workflow using highly specific antibodies pre-coated on a microtiter plate.