Human ANKRD1 (Ankyrin Repeat Domain 1) ELISA Kit (HUES01806)

Beyond its established role as a stretch-responsive transcriptional co-factor in cardiomyocytes, ANKRD1 partners with transcription factors such as Nkx2-5 and MEF2C to drive stress gene programs, and elevated circulating levels have been reported in acute myocardial infarction and chronic heart failure cohorts, supporting its evaluation as a serum biomarker. The Assay Genie sandwich ELISA is calibrated against recombinant human ANKRD1 and tolerates standard sample volumes of 50-100 µL per well, with intra-assay and inter-assay precision typically below 10% CV in published validation runs. Researchers studying pressure-overload hypertrophy, doxorubicin cardiotoxicity, and skeletal muscle regeneration routinely pair this kit with downstream western blot or qPCR readouts to confirm transcript-level changes at the protein level. Reconstituted standards and detection antibody should be kept on ice during use, aliquoted into single-use vials to prevent more than two freeze-thaw cycles, and discarded if the working dilution shows visible turbidity. For research use only.