Human Angiotensin Converting Enzyme 2/ACE2 ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the precise measurement of Angiotensin-Converting Enzyme 2 (ACE2) levels in human samples. Using a double-antibody detection method, it is suitable for quantifying ACE2 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, offering a sensitivity of 0.234 ng/mL and a working range of 0.391–25 ng/mL.

ACE2 is a pivotal enzyme involved in the regulation of the renin-angiotensin system, playing a critical role in cardiovascular health and in the pathogenesis of various diseases, including COVID-19. By measuring ACE2 levels, researchers can gain insights into disease mechanisms and potential therapeutic targets, making this kit a valuable tool for studying ACE2 biology within the immunology research area. Known synonyms include ACEH and ACEII.

The assay uses a colorimetric workflow: standards and samples are incubated on a pre-coated plate, followed by a biotin-labelled detection antibody and HRP-Streptavidin conjugate, with signal developed using TMB substrate and read at 450 nm after adding stop solution. Reported linearity falls between 81–102% across serum, EDTA plasma and heparin plasma dilutions, and recovery averages 96–97% in the same matrices. Reagents are stable at 2–8°C for 12 months.