Human Angiotensin 1-7 (Ang1-7) ELISA Kit

The Assay Genie Angiotensin 1-7 (Ang1-7) ELISA Kit uses a competitive immunoassay format to quantify the heptapeptide hormone Ang1-7, a key effector of the ACE2/Ang-(1-7)/Mas receptor axis that counterbalances the pressor actions of angiotensin II in cardiovascular and renal physiology research. The kit provides a detection range of 15.625 to 1000 pg/mL with a sensitivity below 9.375 pg/mL, requires a 50 uL sample volume per well, and has been validated across multiple biological matrices including tissue homogenates, cell lysates, and cell culture supernatants. Reconstituted standards and pre-titered detection antibody are combined with sample in coated microplate wells, washed, and developed with TMB substrate for an absorbance-based readout. Reagents should be stored refrigerated at 4 degrees C, protected from light, and the kit is intended for research use only in laboratory settings.