Human Angiopoietin-like 3/ANGPTL3 ELISA Kit

Assay Genie offers a sandwich ELISA kit designed for the quantitative measurement of human Angiopoietin-like 3 (ANGPTL3), a protein implicated in lipid metabolism and regulation that is relevant to cardiovascular research. The kit is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples of human origin, and uses a colorimetric detection method in a double-antibody sandwich format.

Reactivity is restricted to human samples, and kit components are intended to remain stable when stored at 2-8°C for 12 months.

Reported linearity for serum across 1:2 to 1:8 dilutions spans 95-104%, with EDTA plasma at 82-100% and heparin plasma at 81-99% (all n=5). Average recoveries are 95% for serum, 94% for EDTA plasma, and 98% for heparin plasma. The procedure is a six-step protocol that ends with TMB color development and absorbance reading at 450 nm.