Human Angiopoietin 2/ANG2 ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the colorimetric detection of Human Angiopoietin 2 (ANG-2) using a double antibody format. The assay is intended for use with human samples, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. It offers a sensitivity of 28.125 pg/mL and a standard measuring range of 46.875–3000 pg/mL. Reagents should be stored at 2–8°C and remain stable for up to 12 months.

Angiopoietin 2 is a key regulator of angiogenesis, the process of forming new blood vessels, and has been linked to cancer, cardiovascular diseases, and neurodegenerative disorders, making it a valuable biomarker for research into these conditions and potential therapies. The kit supports reliable and reproducible quantification of ANG-2 levels through a streamlined workflow: reagents and TMB substrate are equilibrated to room temperature, samples and standards are incubated to allow antigen binding, a biotin-labeled detection antibody and HRP-streptavidin conjugate are applied in sequence, TMB substrate is added for color development, and absorbance is read at 450 nm after addition of stop solution.