Human Angiogenin PharmaGenie ELISA Kit (SBRS0252)

The Assay Genie Human Angiogenin ELISA Kit enables quantitative measurement of angiogenin, a 14 kDa protein belonging to the pancreatic ribonuclease superfamily. Angiogenin was originally purified from conditioned medium of human adenocarcinoma HT-29 cells and is now recognized as a potent mediator of tumor angiogenesis, stimulating endothelial cell proliferation and migration. Clinical research has linked angiogenin to neurodegenerative conditions, with mutations in the ANG gene associated with familial ALS and Parkinson's disease. The kit specifically detects the human isoform, supporting translational oncology studies, wound healing investigations, and neurological disease biomarker research. Standards, capture antibodies, and detection reagents are supplied in a microplate format covering 96 determinations, and the chromogenic read-out is compatible with standard plate readers. Sample volume per well is typically 100 µL, and reconstituted standards should be aliquoted and stored frozen to limit freeze-thaw cycles. Researchers studying tumor vascularization, cerebral ischemia, or placental angiogenesis will find the kit relevant for profiling angiogenic activity across cell culture supernatant and circulating blood fractions.