Human and Mouse Phospho-ULK1 (S556) PharmaGenie ELISA Kit (SBRS2011)

This phosphorylation ELISA from Assay Genie is built to quantify ULK1 protein when phosphorylated at the serine 556 residue, a regulatory site targeted by AMPK within the autophagy induction cascade. The assay enables researchers studying mTOR/AMPK signaling, nutrient sensing, and cellular stress responses to measure phospho-ULK1 S556 levels in cell lysates and tissue lysates with site-specific selectivity. Compatible samples include lysates prepared from cultured mammalian cells and snap-frozen tissues, typically generated with RIPA-based buffers supplemented with phosphatase inhibitors to preserve the phosphorylation mark; the standard protocol loads 100 µL of sample per well. Each kit provides pre-coated microplate strips along with a phosphorylation-specific detection antibody, recombinant phospho-protein standards, and TMB substrate buffer sufficient for one full plate run. Reagents are best stored at 4°C for short-term use or aliquoted and frozen at -20°C for longer preservation; avoid repeated freeze-thaw cycles of the standard and antibody vials. Intended for research use only, supporting autophagy, metabolism, and kinase signaling studies, including investigations of cancer cell survival, neurodegeneration, and metabolic disease models.