Human and Mouse Phospho-ULK1 (S556) and Total ULK1 PharmaGenie ELISA Kit (SBRS2012)

ULK1 (Unc-51 Like Autophagy Activating Kinase 1) is a serine/threonine kinase that initiates autophagy through phosphorylation of downstream substrates such as Beclin-1 and ATG13. Phosphorylation at Ser556 is a regulatory mark linked to mTOR-dependent autophagy control, making quantitative measurement of phospho-ULK1 alongside total ULK1 valuable for studies of nutrient sensing, cellular stress, and mTORC1 signaling. This dual-target ELISA from Assay Genie enables simultaneous immunocapture of both the S556-phosphorylated and total forms of ULK1 from a single lysate preparation, supporting normalization of phosphorylation status against total protein expression. The kit is validated on cell lysates and tissue lysates of human and mouse origin, allowing cross-species comparison in translational autophagy research.

Typical workflow involves lysing cells or tissues in the presence of phosphatase inhibitors to preserve the S556 phosphorylation mark, loading clarified lysate into the pre-coated microwell plate, and reading absorbance after chromogenic substrate addition. For storage, the unopened kit should be kept refrigerated at 4 degrees C; once opened, individual components can be aliquoted and frozen to limit repeated freeze-thaw cycles. Researchers commonly apply this assay to investigate AMPK/mTOR pathway crosstalk, starvation- or exercise-induced autophagy flux, and pharmacologic modulation of kinase signaling in disease models, and it is supplied for research use only.