Human and Mouse Phospho-SHP1 (S591) PharmaGenie ELISA Kit (SBRS1964)

Assay Genie's Phospho-SHP1 (S591) PharmaGenie ELISA Kit targets the S591 phosphorylation site of SHP-1, a Src homology 2 domain-containing protein tyrosine phosphatase (also called PTPN6 or SH-PTP1) that modulates signaling downstream of cytokine receptors, the B-cell receptor, and the T-cell receptor in hematopoietic cells. The assay quantitatively measures serine 591 phosphorylation in cell lysates and tissue lysates from human and mouse samples, supporting cross-species comparison of phosphatase pathway activity in immunology, hematopoiesis, and oncology research.

Each kit contains a pre-coated microplate and reagents for a colorimetric immunoassay workflow calibrated for 96 determinations. Researchers load clarified lysate at a small microliter sample volume per well, generate a phospho-SHP1 (S591) standard curve, and read absorbance to interpolate phosphorylation levels across biological replicates. The format is for research use only and is not cleared for clinical diagnostic procedures.

Common applications include tracking SHP-1 pathway activation following cytokine or growth factor stimulation, mapping phosphatase regulation in leukemia and lymphoma cell models, and confirming site-specific phosphorylation events observed in western blot experiments. Store kit components refrigerated at 4 degrees C and protect from light; refer to the lot manual for individual component storage and shelf life information.