Human and Mouse Phospho-PLCG2 (Tyr753) PharmaGenie ELISA Kit (SBRS1934)

The Phospho-PLCG2 (Tyr753) PharmaGenie ELISA Kit from Assay Genie enables quantitative measurement of tyrosine 753 phosphorylation on phospholipase C gamma 2, a key node in B-cell receptor and Fc receptor signal transduction cascades. Researchers apply this phosphorylation ELISA to probe pathway activation downstream of antigen receptors in immune cells, with validated reactivity against both human and mouse PLCG2.

The assay runs on a pre-coated microplate and accepts cell lysates or tissue lysates as input material, supporting stimulation time-course experiments in lymphocyte and mast cell models. Tyrosine 753 phosphorylation reflects PLCG2 activation state, which is widely studied in B-cell malignancies, immune deficiency syndromes, and autoimmunity research.

The phospho-specific capture reagent selectively recognizes the Tyr753 epitope rather than total PLCG2 protein, allowing direct comparison of basal versus stimulated signaling in cultured cells or snap-frozen tissue.