Human and Mouse Phospho-FRS2 (Y436) and Total FRS2 PharmaGenie ELISA Kit (SBRS1836)

Built on the Phospho-FRS2 (Y436) and Total FRS2 dual-target design from Assay Genie, this phosphorylation ELISA enables researchers to quantify tyrosine-436 phosphorylation of fibroblast growth factor receptor substrate 2 (FRS2), an approximately 80 kDa membrane-anchored adaptor, alongside total FRS2 protein levels in a single workflow. FRS2 links activated FGFR and RET receptor tyrosine kinases to downstream MAPK and PI3K/AKT cascades, and Y436 is one of four critical phospho-tyrosine residues required for Grb2-mediated signal propagation. The assay is validated for cell lysates and tissue lysates derived from human and mouse samples, supporting studies of receptor tyrosine kinase signaling, neuronal development, and oncology research models where aberrant FGFR pathway activity is implicated. The kit provides a quantitative tool for monitoring pathway activation or inhibition in response to pharmacological agents, genetic perturbations, or ligand stimulation. Shipped refrigerated with all components ready to load, the ELISA uses a microplate protocol suitable for research use only in laboratory settings, with a sample volume typically around 100 uL per well.