Human and Mouse Phospho-EGFR (Y992) and Total EGFR PharmaGenie ELISA Kit (SBRS1798)

Phosphorylation studies on the epidermal growth factor receptor gain a quantitative, dual-analyte readout with an immunoassay that captures phosphorylated EGFR at tyrosine 992 alongside total EGFR receptor abundance from matched samples. Produced by Assay Genie, the kit is configured for 96 assays in a plate-based workflow that runs on standard absorbance readers and accepts cell lysates and tissue lysates as input. By resolving phospho-Y992 signal in parallel with total receptor levels, the workflow supports normalization of activation state against receptor expression, a comparison widely applied in receptor tyrosine kinase characterization, oncology research, and small-molecule inhibitor profiling.

The lysate-based input is compatible with standard lysis buffer preparations commonly used for Western blot and immunoprecipitation workflows, allowing straightforward integration into existing cell-signaling pipelines. With Human reactivity, the assay accommodates patient-derived samples, established cell-line panels, and xenograft-derived tissue. Each ELISA plate supplies the reagents required for the dual phospho and total measurement, simplifying benchmarking of EGFR pathway activity across treatment conditions, time-course experiments, and matched sample cohorts.