Human and Mouse Phospho-EGFR (Y1086) PharmaGenie ELISA Kit (SBRS1795)

Phospho-EGFR (Y1086) is a major autophosphorylation site on the Epidermal Growth Factor Receptor that serves as a docking site for PLC-gamma and couples EGF binding to downstream MAPK and PI3K/AKT signaling. Quantifying phosphorylation at this residue is widely used in oncology research, including studies of non-small cell lung cancer, glioblastoma, and head and neck carcinoma where EGFR dysregulation drives proliferation and resistance to tyrosine kinase inhibitors.

The kit from Assay Genie is configured for a 96-well plate immunoassay format and uses a phosphorylation site-specific antibody pair to measure phospho-Y1086 in cell lysates and tissue lysates. It has been validated for cross-reactivity with both human and mouse EGFR, supporting comparative studies across human tumor samples and corresponding murine disease models. Reconstituted standards and detection antibodies should be aliquoted and stored frozen to preserve activity through repeated freeze-thaw cycles, while the microplate and wash buffer remain stable under refrigerated storage. Researchers typically load 100 uL of lysate per well, making the assay compatible with modest sample inputs from cultured cells and small biopsy preparations in pathway profiling and inhibitor screening workflows.