Human Amyloid beta A4 protein (APP) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the quantitative measurement of human Amyloid beta A4 protein (APP) in human biological samples, providing a tool for researchers investigating Alzheimer's disease and other neurodegenerative disorders. Amyloid beta is recognised as a key toxic peptide that accumulates in the brain, leading to the formation of plaques and neuronal damage, so detecting and quantifying its levels can offer insights into disease progression and potential therapeutic targets.

The assay is reactive with human samples and has been validated for use with serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. Specificity is reported for natural and recombinant human Amyloid-beta precursor protein. Reported performance characteristics include a detection range of 15.6-1000pg/mL and a sensitivity of 10pg/mL, with an intra-assay CV of 5.3% and an inter-assay CV of 10.1%.

Linearity has been evaluated in serum, EDTA plasma and heparin plasma across dilutions of 1:2, 1:4, 1:8 and 1:16, and recovery has been assessed in serum and plasma. The kit is supplied for research use only, falls within the Neurosciences research area, and targets the protein catalogued under UniProt P05067.