Human Amylase Alpha ELISA Kit (HUFI04786)

Alpha-amylase (EC 3.2.1.1) is a calcium-dependent hydrolytic enzyme secreted mainly by salivary glands and the exocrine pancreas, and circulating concentrations are widely used biomarkers for pancreatic injury, salivary gland inflammation, and abdominal emergencies. The Assay Genie Human Amylase Alpha ELISA Kit is a quantitative immunoassay that supports research studies of this enzyme in human samples.

This colorimetric assay is supplied in a 96 Assays configuration and is intended for research use only. It supports laboratories investigating gastroenterology, oral physiology, pancreatic biology, and biomarker discovery, particularly when quantitative comparison across patient cohorts is required.

For proper handling, kit components should be refrigerated upon arrival and equilibrated to room temperature before use. Protect the substrate solution from prolonged light exposure to preserve color development. Reconstituted standards and working antibody solutions should be aliquoted to limit repeated freeze-thaw cycles, which can degrade binding activity.

Each kit includes a pre-coated microplate, detection antibody, calibrated standards, wash buffer, and TMB substrate, providing everything needed to generate a full standard curve and quantify alpha-amylase in research samples.