Human AmiGO2 PharmaGenie ELISA Kit (SBRS0250)

The Assay Genie Human AmiGO2 PharmaGenie ELISA Kit enables quantitative measurement of human AMIGO2 (Adhesion Molecule with Ig-like Domain 2), a transmembrane cell-surface protein implicated in neuronal development, angiogenesis, and tumor progression research. The human-specific capture antibody distinguishes AMIGO2 across multiple sample matrices without significant cross-reactivity to homologous family members AMIGO1 or AMIGO3, supporting studies in neurobiology and oncology where AMIGO2 expression has been linked to cancer cell adhesion, vascular remodeling, and metastatic behavior.

Each kit contains a pre-coated microplate, biotin-conjugated detection antibody, streptavidin-HRP conjugate, calibrated recombinant AMIGO2 standards, and assay buffer sufficient for 96 determinations. Researchers typically load 100 uL of prepared sample and standard per well, run the standard curve alongside unknowns, and read absorbance to interpolate AMIGO2 concentration. Store at 4 degrees C; protect the streptavidin-HRP conjugate from light to preserve enzymatic activity. For research use only, not intended for diagnostic procedures.