Human Alpha-S1-casein (CSN1S1) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the precise measurement of Human Alpha-S1-casein (CSN1S1) levels in human serum, plasma, and cell culture supernatants. CSN1S1 is a key component of milk protein that contains bioactive peptides with potential health benefits, and research has studied its reported anti-inflammatory, immunomodulatory, and antioxidant properties in the context of conditions such as diabetes, obesity, and cardiovascular diseases. The kit is for research use only.

Performance characteristics include a detection range of 78-5000pg/mL and a sensitivity of 42.6pg/mL. Intra-assay CV is reported as 6.5% and inter-assay CV as 10.1%. Linearity has been established for serum, EDTA plasma, and heparin plasma across dilutions of 1:2, 1:4, 1:8, and 1:16. Recovery averages 106% (range 100-112%) in serum and 108% (range 102-114%) in plasma.

The kit is specific for natural and recombinant human Alpha-S1-casein (synonyms: CASA, CSN1; UniProt P47710). Alpha-S1-casein plays an important role in the capacity of milk to transport calcium phosphate, and Casoxin D derived from it acts as an opioid antagonist with vasorelaxing activity mediated by bradykinin B1 receptors. Reported molecular weight is 20,693 Da and NCBI Gene ID is 1446. Detection is performed using a colorimetric ELISA method.