Human alpha-MSH ELISA Kit

A high-quality competitive ELISA from Assay Genie is designed to accurately detect levels of Alpha-MSH (Melanocyte-Stimulating Hormone) in human samples. The assay works with serum, plasma, cell culture supernatants, cell or tissue lysates, and other liquid sample types, and is suited to research into pigmentation regulation, immune response, metabolism, melanocyte function, immune system regulation, and metabolic disorders. Alpha-MSH is highlighted as a potential biomarker linked to various skin conditions, autoimmune disorders, and metabolic diseases.

Performance characteristics include a sensitivity of 7.5 pg/mL and a working range of 12.5-800 pg/mL. Linearity across dilutions (1:2, 1:4, 1:8) is reported as 86-102% / 92-99% / 95-103% for serum (n = 10), 82-91% / 82-100% / 87-96% for EDTA plasma (n = 10), and 80-94% / 84-99% / 82-89% for heparin plasma (n = 10). Recovery ranges and averages are 87-99% (92%) for serum (n = 10), 88-105% (95%) for EDTA plasma (n = 10), and 87-105% (97%) for heparin plasma (n = 10). The assay is read at 450 nm following TMB color development, and the kit should be stored sealed at 2-8°C and not cryopreserved.

The protocol involves equilibrating TMB substrate, pre-washing the plate, adding standard or sample with biotin-labelled antibody, incubating at 37°C, washing, developing with TMB in the dark, stopping the reaction, and measuring absorbance. Sample preparation guidance covers serum, plasma, cell culture supernatant, cell lysate, tissue homogenate, and other liquid sample types. The kit is supplied with a dismountable ELISA microplate, lyophilized standard, lyophilized biotin-labelled antibody, HRP-Streptavidin conjugate, TMB substrate, purified water, sample, antibody and SABC dilution buffers, stop solution, wash buffer, plate sealer, and a technical manual.