Human Alpha-Endorphin (aEP) ELISA Kit

The competitive assay format from Assay Genie is calibrated for human alpha-endorphin with minimal cross-reactivity to homologous opioid peptides, making it well-suited for studies of pain modulation, HPA axis activity, and pituitary-derived neuroendocrine signaling. The microplate-based workflow accommodates serum, plasma, tissue homogenates, and cell culture supernatants, with competitive antibody reagents, lyophilized standards, and detection buffers supplied. Reagent components are stored refrigerated and should be brought to room temperature before running the protocol. As a 16-amino acid peptide processed from proopiomelanocortin (POMC), alpha-endorphin is most readily quantified by competitive immunoassay rather than sandwich format, which requires two non-overlapping epitopes.