Human Alpha-1-Microglobulin/Bikunin Precursor (a1M) ELISA Kit

A solid-phase microplate ELISA built for quantitative measurement of Alpha-1-microglobulin/Bikunin Precursor (AMBP), also called protein HC or a1M, in human research specimens. This complete 96-assay test kit, distributed by Assay Genie, supplies a pre-coated capture plate, paired detection antibodies, lyophilised reference standards, chromogenic substrate, wash buffer, and stop solution in a ready-to-run format.

Each plate well is pre-coated with capture antibodies specific for the AMBP precursor. Bound analyte is revealed by a paired antibody conjugated to horseradish peroxidase; the resulting chromogenic reaction is read on a standard microplate reader using absorbance detection, and signal intensity correlates with a1M concentration. Because the two-site interaction requires intact paired binding, the format can distinguish the precursor and its free alpha-1-microglobulin subunit from bikunin-linked complexes, useful for dissecting the two physiological roles encoded by a single gene.

The AMBP gene on chromosome 9 directs synthesis of a precursor polypeptide that, after proteolytic cleavage, releases alpha-1-microglobulin (a lipocalin-family carrier implicated in heme binding and handling of oxidative stress) and bikunin (a Kunitz-type serine protease inhibitor with anti-inflammatory activity). Because urinary a1M rises with proximal tubule dysfunction, the marker is widely used in clinical research on renal handling of low-molecular-weight proteins, while plasma measurements inform studies of pregnancy, hepatic synthesis, redox balance, and acute-phase responses. Validated for in vitro research use only, this kit accommodates a broad set of human sample matrices, from urinary specimens and blood-derived fractions, including serum-type and plasma-type samples, to other body fluids, supporting flexible study design.