Human Alpha-1-acid glycoprotein ELISA Kit

A high-quality Sandwich ELISA Kit from Assay Genie designed for the precise measurement of alpha-1-acid glycoprotein levels in human biological samples. The assay uses a colorimetric detection method in a double-antibody sandwich format, with a reported sensitivity of 0.938 ng/mL and a detection range of 1.563–100 ng/mL.

The kit is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and is intended for research applications within the immunology field. According to Assay Genie, alpha-1-acid glycoprotein is a vital protein that plays a key role in the immune response and inflammation processes, and its levels have been linked to conditions such as inflammatory diseases, cancer, and infectious diseases, making it a valuable biomarker for studying these disorders.

The assay workflow involves equilibrating reagents to room temperature, incubating standards and samples on the pre-coated plate at 37 °C, binding with a biotin-labelled detection antibody, adding HRP-Streptavidin, developing colour with TMB substrate in the dark, stopping the reaction, and reading absorbance at 450 nm. Kit components should be stored at 2–8 °C for 12 months.