Human ALKBH1 ELISA Kit (HUFI04655)

AlkB Homolog 1 (ALKBH1) is a nucleic acid demethylase and DNA alkylation repair enzyme, and this 96 Assays ELISA Kit from Assay Genie provides researchers with a quantitative immunoassay workflow to measure ALKBH1 protein abundance in human-derived specimens. As a Fe(II)/2-oxoglutarate-dependent dioxygenase, ALKBH1 contributes to the oxidative reversal of N6-methyladenine on DNA and other alkylated nucleobases, linking its activity to epigenetic regulation and maintenance of genomic integrity.

Each kit includes the principal reagents required to perform the assay: pre-coated microwell plate, lyophilized recombinant standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, substrate, and stop solution. The microplate is read on a standard absorbance plate reader, integrating easily with most laboratory instrumentation.

Storage and handling recommendations include refrigerating the kit between uses, equilibrating all components to room temperature before initiating the protocol, preparing the standard curve fresh for each run, and limiting freeze-thaw cycles of the standards and conjugate to preserve binding activity. This product is intended for research use only.